Venue: Feyenoord Stadium Date: Sunday, 1 June Kick-off: 1400 BST
Coverage: Live commentary on BBC Radio Wales, BBC Radio Cymru and Online. Highlights on Match of the Day Wales at 2000 BST on BBC Two Wales
Dutch star Ryan Babel has been ruled out of Sunday's Wales friendly and the Euro 2008 finals with an ankle injury.
The Liverpool attacker tore ankle ligaments in training on Saturday and coach Marco van Basten is considering who will replace Babel in his squad.
John Toshack has confirmed captain Craig Bellamy will not start in Rotterdam as Carl Robinson could become the Wales manager's eighth skipper.
Wales are also without midfielders Carl Fletcher and Owain Tudur Jones.
Defender Craig Morgan has a foot problem and faces a late fitness test but one man who will miss Sunday's game is Robin van Persie.
The injury-plagued Arsenal striker has not fully recovered from a thigh injury and van Basten is reluctant to risk one of his star strikers on the eve of the European Championships.
Van Persie was van Basten's top-scorer during the Euro 2008 qualifiers and the Dutch coach admits: "We're not going to take any risks with Robin, because he's a special player who'll be very useful when we face Italy on 9 June."
Bellamy, too, has endured a season of injury torment but the West Ham striker is almost certain to be in Wales' matchday squad on Sunday as a substitute.
His 30 minute cameo appearance during the 1-0 win in Iceland was his first game for virtually seven months.
Fletcher and Tudur Jones have both flown home with ankle injuries as Wales try to extend an impressive three-match winning streak in the De Kuip Stadium.
Toshack gave three of his teenagers their Wales bow against Iceland and Manchester City rookie Ched Evans shone with a match-winning display performance while fellow striker Sam Vokes showed flashes of his huge promise.
And Cardiff City's highly-rated 17-year-old Aaron Ramsey is set to earn his international stripes inside a packed 50,000 Rotterdam cauldron.
"Holland will be one of the favourites for Euro 2008," acknowledged Toshack.
"So we know this will be a big test for our young players.
"It will be the biggest game some of our lads will have played in."
Not Ramsey's biggest game, though, whose impressive cameo appearance in Cardiff's FA Cup final defeat to Portsmouth just consolidated his reputation as the most-wanted player outside the Premier League.
Manchester United, Arsenal and Liverpool are all ready to pay £10m for the young Wales playmaker and another composed and sublime display in Rotterdam would add to his value still further.
With Bellamy on the bench, Toronto midfielder Robinson - who is poised to captain Wales for the first time - will be Wales' most experienced starter in Rotterdam by far should he win his 46th cap.
Toshack's talisman Jason Koumas, who'll win his 29th cap, will be next. A startling 22 of Toshack's 30-man squad available to face the Dutch have ten caps or less.
It should be men against boys as young Wales face the technically gifted original architects of total football.
Toshack admits: "It will be interesting to see how they do but when you look at the quality of players we are facing, it is going to be very hard.
"What pleases me is that the younger players ensure we have a much, much deeper squad than what we had.
"In my first game as Wales manager against Hungary back in 2005 I struggled to get 16 or 17 players for that squad.
"We've got a good group of young talent who are not ready yet but I feel we're on the right lines.
"And with all modesty, which hasn't always been my strong points, we haven't done that bad as in a dozen games we've had four wins, six draws and two defeats to Cyprus and Germany.
"We've had to reconstruct things and under difficult circumstances I'm pleased how things have gone."
The Netherlands' Euro 2008 squad: Edwin van der Sar (Manchester United), Maarten Stekelenburg (Ajax Amsterdam), Henk Timmer (Feyenoord), Wilfred Bouma (Aston Villa), Tim de Cler (Feyenoord), John Heitinga (Ajax Amsterdam), Joris Mathijsen (Hamburg SV), Mario Melchiot (Wigan Athletic), Andre Ooijer (Blackburn Rovers), Ibrahim Afellay (PSV Eindhoven), Giovanni van Bronckhorst (Feyenoord), Orlando Engelaar (Twente Enschede), Nigel de Jong (Hamburg SV), Wesley Sneijder (Real Madrid), Rafael van der Vaart (Hamburg SV), Demy de Zeeuw (AZ Alkmaar), Klaas Jan Huntelaar (Ajax Amsterdam), Dirk Kuyt (Liverpool), Ruud van Nistelrooy (Real Madrid), Robin van Persie (Arsenal), Arjen Robben (Real Madrid), Jan Vennegoor of Hesselink (Celtic).
Wales squad: Wayne Hennessey (Wolves), Boaz Myhill (Hull City), Lewis Price (Derby County), Darcy Blake (Cardiff City), Neal Eardley (Oldham Athletic), Steve Evans (Wrexham), Chris Gunter (Tottenham Hotspur), Joe Jacobson (Bristol Rovers), Craig Morgan (Peterborough United), Lewin Nyatanga (Derby County), Sam Ricketts (Hull City), Ashley Williams (Swansea City), Jack Collison (West Ham), Andrew Crofts (Gillingham), Arron Davies (Nottingham Forest), David Edwards (Wolves), Mark Jones (Wrexham), Jason Koumas (Wigan Athletic), Joe Ledley, Aaron Ramsey (both Cardiff City), Carl Robinson (Toronto), Craig Bellamy (West Ham), Craig Davies (Oldham Athletic), Freddy Eastwood (Wolves), Ched Evans (Manchester City), Daniel Nardiello (QPR), Sam Vokes (Wolves).